Table 1.
miR-138 targets identified by EMT-specific qRT-PCR array and bioinformatics prediction
| Transcripts down-regulated by miR-138 treatment (qRT-PCR) |
miR-138 targets predicted by bioinformatic tools |
|||||||
|---|---|---|---|---|---|---|---|---|
| Gene name | Fold difference (miR-138/control) | P value | PicTar 4-way | PicTar 5-way | TargetScanS | TargetScan Human 5.1 | miRanda (microrna.org) | miRanda (miRBase) |
| EZH2 | 0.24 | 0.001 | 1 | 1 | 1 | 1 | 1 | |
| SERPINE1 | 0.31 | 0.009 | ||||||
| PLEK2 | 0.32 | 0.065 | ||||||
| ZEB2 | 0.38 | 0.022 | 1 | |||||
| SNAI2 | 0.43 | 0.001 | ||||||
| MMP3 | 0.43 | 0.001 | ||||||
| RHOC | 0.44 | 0.008 | 1 | 1 | 1 | 1 | ||
| VIM | 0.48 | 0.005 | 1 | 1 | 1 | |||
| CDH2 | 0.49 | 0.078 | ||||||
The expressional changes for the 86 EMT-related genes we examined are shown in Supplementary Table S1 at at http://www.BiochemJ.org/bj/440/bj4400023add.htm. Genes that were predicted to be candidate targets of miR-138 were identified by the number 1 Gene names in bold have a fold difference < 0.5 (P < 0.10) and are bioinformatically predicted to be miR-138 targets. The down-regulation of EZH2 by miR-138 has recently been demonstrated in chicken model [6]. The down–regulation of the RHOC gene by miR-138 has been experimentally confirmed in our recent study [12]. CDH2, cadherin 2; MMP3, matrix metallopeptidase 3; PLEK2, pleckstrin 2.