FIG. 3.

Loss of AMPK-α2 hampers the CR-induced activation of the IRS-1–Akt signal pathway. A: IRS-1 was enriched by immunoprecipitation and then probed with anti–phospho-Tyr antibody to detect the Tyr phosphorylation in skeletal muscle of mice. B–D: In skeletal muscle of mice upon insulin stimulation, the influences of CR on phosphorylation of Akt (B), TBC1D1 (C), and TBC1D4 (D) were impaired by deletion of AMPK-α2. **P < 0.01 vs. WT-AL, #P < 0.05 vs. WT-CR. n = 4 per group. α2KO, AMPK-α2 knockout mice; IP, immunoprecipitation; IB, immunoblot.