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. 2012 Apr 13;61(5):1143–1152. doi: 10.2337/db11-1154

FIG. 1.

FIG. 1.

Analysis of HGF and c-Met expression in islets from pregnant mice at GD15. A: Western blot analysis of HGF expression in protein extracts of islets isolated from nonpregnant (NP) and pregnant female wild-type mice at GD15. Results are means ± SEM of nonpregnant (n = 3) and pregnant (n = 3) mice. *P < 0.05 vs. nonpregnant mouse islets. B: Serum HGF levels in nonpregnant or pregnant female wild-type mice at GD15 and -19 measured by ELISA. Results are means ± SEM of nonpregnant (n = 3) and pregnant (n = 5) mice. *P < 0.05 vs. nonpregnant mice. Real-time PCR analysis of c-Met mRNA expression in total RNA (C) and Western blot analysis of c-Met expression in protein extracts from islets obtained from nonpregnant and pregnant wild-type mice at GD15 (D). Results are means ± SEM of nonpregnant (n = 3) and pregnant (n = 3) mice. *P < 0.05 vs. nonpregnant mouse islets. E: Real-time PCR analysis of c-Met mRNA expression in total RNA extracted from islets treated in vitro for 24 h with 50 nmol/L progesterone (PRG), 10 nmol/L 17β-estradiol (E2), 50 ng/mL PRL, or 200 nmol/L dexamethasone (DEX). Results are means ± SEM of n = 4–8 samples analyzed per condition. *P < 0.05 vs. untreated (C) mouse islets. HPRT, hypoxanthine-guanine phosphoribosyltransferase.

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