FIG. 4.

Pregnant PancMet KO mouse islets displayed altered levels and/or cellular localization of markers involved in cell cycle progression in β-cells. A: Real-Time PCR analysis of PRLR mRNA expression in total islet RNA from islets isolated from nonpregnant (NP) and pregnant (GD15) wild-type (WT) (n = 3–4) and PancMet KO (n = 4–5) mice. Results are means ± SEM. *P < 0.05 vs. nonpregnant mouse islets of their corresponding genotype; ^P < 0.05 vs. wild type at the same GD. HPRT, hypoxanthine-guanine phosphoribosyltransferase. B: Western blot of PRLR in islet protein extracts from nonpregnant and pregnant (GD15) wild-type (n = 4) and PancMet KO (n = 4) mice. Results are means ± SEM. ^P < 0.05 vs. wild type at the same GD. C: Representative photomicrographs of pregnant (GD15) wild-type and PancMet KO mouse pancreatic sections stained for insulin (green) and STAT5 (red). Notice the nuclear localization of STAT5 in the wild-type islet that is absent in the PancMet KO islet. D: Real-time PCR analysis of FoxM1 mRNA expression in total islet RNA from islets isolated from nonpregnant and pregnant (GD15) wild-type (n = 3–4) and PancMet KO (n = 4–5) mice. Results are means ± SEM. *P < 0.05 vs. nonpregnant mouse islets of their corresponding genotype; ^P < 0.05 vs. wild type at the same GD. E: Western blot analysis of p27 expression in islet protein extracts from nonpregnant and pregnant (GD15) wild-type (n = 4–5) and PancMet KO (n = 4–5) mice. Results are means ± SEM. *P < 0.05 vs. nonpregnant mouse islets of their corresponding genotype; ^P < 0.05 vs. wild type at the same GD. F: Representative photomicrographs of pregnant (GD15) wild-type and PancMet KO mouse pancreatic sections stained for insulin (green) and p27 (red). Notice the increased intensity of staining of p27 in the PancMet KO islet. (A high-quality digital representation of this figure is available in the online issue.)