FIG. 5.

Increased β-cell death in pregnant PancMet KO mice at GD15. A and B: Representative photomicrographs of islets from pregnant PancMet KO mice and wild-type (WT) littermates at GD15 stained for insulin (red) and TUNEL (green). Arrows indicate TUNEL-positive β-cell nuclei. C: Quantification of the percentage of TUNEL-positive β-cells in pregnant, PPD4, and nonpregnant (NP) wild-type (n = 4–8) and PancMet KO (n = 4–7) mice. Results are means ± SEM. *P < 0.05 vs. nonpregnant mice of their corresponding genotype; ^P < 0.05 vs. wild type at the same GD or PPD4. D–I: Representative photomicrographs of mouse islet cultures from wild-type (D–F) and PancMet KO (G–I) mice treated with 0 nmol/L (D and G), 200 nmol/L (E and H), or 500 nmol/L (F and I) dexamethasone for 24 h and stained for TUNEL (green), insulin (red), and DAPI (blue). Arrows indicate TUNEL-positive β-cell nuclei. J: Quantification of TUNEL-positive β-cell nuclei in four experiments performed in duplicate of mouse islet cell cultures of PancMet KO and wild-type mice treated with 0, 200, or 500 nmol/L dexamethasone. Results are means ± SEM. *P < 0.05 vs. 0 mmol/L of their corresponding genotype; ^P < 0.05 vs. the same dose in wild-type mouse islet cell cultures. K–N: Representative photomicrographs of human islet cultures treated with 0 ng/mL (K and L) or 25 ng/mL (M and N) HGF and 0 nmol/L (K and M) or 500 nmol/L (L and N) dexamethasone for 24 h and stained for TUNEL (green), insulin (red), and DAPI (blue). Arrows indicate TUNEL-positive β-cell nuclei. O: Quantification of TUNEL-positive β-cell nuclei in three experiments performed in duplicate of human islet cell cultures untreated or treated with HGF and 0 or 500 nmol/L dexamethasone. Results are means ± SEM. *P < 0.05 vs. 0 nmol/L and ^P < 0.05 vs. 500 nmol/L without HGF. (A high-quality digital representation of this figure is available in the online issue.)