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. 2012 Apr 13;61(5):1143–1152. doi: 10.2337/db11-1154

FIG. 6.

FIG. 6.

Glucose homeostasis in pregnant PancMet KO mice. Pregnant PancMet KO mice displayed significantly increased blood glucose (A) and significantly decreased plasma insulin (B) at GD19. Results are means ± SEM and were obtained from pregnant, PPD4, and nonpregnant (NP) wild-type (WT) (n = 4–8) and PancMet KO (n = 4–7) mice. *P < 0.05 vs. nonpregnant mice of their corresponding genotype; ^P < 0.05 vs. wild type at the same GD. C: Intraperitoneal glucose tolerance test in wild-type (n = 7) and PancMet KO (n = 6) mice at GD19. Results are means ± SEM. ^P < 0.05 vs. wild type at the same time point. D: Area under the curve (AUC) calculated from the intraperitoneal glucose tolerance test experiments in which nonpregnant and pregnant wild-type (n = 5–7) and PancMet KO (n = 6–8) mice at GD15 and -19 and PPD4 were examined. Results are means ± SEM. *P < 0.05 vs. nonpregnant mice of their corresponding genotype; ^P < 0.05 vs. wild type at the same GD or PP day. E: Insulin tolerance test in pregnant wild-type (n = 5) and PancMet KO (n = 6) mice at GD18. Both types of mice show similar response to insulin administration. F: Real-time PCR analysis of glucokinase, GLUT2, Pdx-1, and insulin mRNA expression in total islet RNA from islets isolated from nonpregnant and pregnant (GD19) wild-type (n = 5–6) and PancMet KO (n = 3–5) mice. Results are means ± SEM. *P < 0.05 vs. wild-type nonpregnant; ^P < 0.05 vs. wild-type GD19. HPRT, hypoxanthine-guanine phosphoribosyltransferase. G: Insulin content in islets isolated from nonpregnant and pregnant (GD19) wild-type (n = 6) and PancMet KO (n = 6) mice. Results are means ± SEM. *P < 0.05 vs. wild-type nonpregnant; ^P < 0.05 vs. wild-type GD19. H: GSIS was performed in groups of 10 islets of similar sizes obtained from nonpregnant and pregnant (GD19) wild-type (n = 5) and PancMet KO (n = 5) mice and incubated for 30 min with 5 or 20 mmol/L glucose. Results are means ± SEM. *P < 0.05 vs. 5 mmol/L glucose of their corresponding genotype; +P < 0.05 vs. nonpregnant wild type at 20 mmol/L glucose; and ^P < 0.05 vs. wild-type GD19 at 20 mmol/L glucose.

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