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. 2012 Apr 13;61(5):1153–1159. doi: 10.2337/db11-1271

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© 2012 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. See http://creativecommons.org/licenses/by-nc-nd/3.0/ for details.

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FIG. 5. — Survival of MSCs modified with genes and secretion of VEGF and FGF-2 by transplanted MSCs in diabetic ischemic hindlimb. A: The GFP-positive MSCs (per 1,000 nuclei) in ischemic muscles of the PGC-1α-MSC group 5 days after transplantation were significantly higher than those in the GFP-MSC group. *P < 0.001. B: Western blotting analysis for VEGF, FGF-2, and GAPDH 5 days after transplantation. C: Secretion of FGF-2 and VEGF by transplanted MSCs that were modified with genes. Colocalization of GFP-positive MSCs (green) with VEGF (red) or GFP-positive MSCs (green) with FGF-2 (red) in ischemic muscles 5 days after transplantation. Scale bars, 10 μm. (A high-quality color representation of this figure is available in the online issue.)