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. 2012 Apr 13;61(5):1062–1071. doi: 10.2337/db11-1255

FIG. 2.

FIG. 2.

Effects of glucoregulatory hormones/factors on the 5′-promoter activity of the PFK2/FBP2 gene. A: Time-course and dose-response effects of insulin. HuH7 cells were transfected transiently with the PFK2/FBP2-Luc plasmid and insulin receptor expression plasmid and then treated with insulin (10 nmol/L) for various time intervals (6–48 h) or treated with various doses of insulin (100 pmol/L to 100 nmol/L) for 36 h. B: Time-course effects of forskolin and glucocorticoid. HuH7 cells were transfected transiently with the PFK2/FBP2-Luc plasmid and glucocorticoid receptor expression plasmid (for glucocorticoid experiment) and then treated with forskolin (10 μmol/L) or dexamethasone (100 nmol/L) for up to 48 h. Each value is shown as a percentage of the control (vehicle, open bars; 0) or value at time zero (open bars, 0). In all figures, closed bars represent values of treated groups. *P < 0.05 vs. corresponding control.