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. 2012 Apr 13;61(5):1062–1071. doi: 10.2337/db11-1255

FIG. 3.

FIG. 3.

Effects of insulin/glucose-related transcription factors on the 5′-promoter activity of the PFK2/FBP2 gene. A: Coexpression experiment. HuH7 cells were transfected with the PFK2/FBP2-Luc and each test plasmid [SREBP1c (S), LXRα/β (Lα/Lβ), and/or RXRα (Rα) and ChREBP (Ch) and/or Mlx expression plasmids] [reporter plasmid (μg): expression plasmid(s) (μg) = 1:1], and then the cells were cultured for 24 h. Each value is shown as a percentage of the corresponding control. *P < 0.05 vs. corresponding control [C (open bars); empty plasmid]. B: Time-course and dose-response experiments. HuH7 cells were transfected with PFK2/FBP2-Luc plasmid and LXRα/RXRα expression plasmids [reporter plasmid (μg): expression plasmids (μg) = 1:1] (time-course study) or PFK2/FBP2-Luc plasmid and increasing amounts of LXRα/RXRα expression plasmids [reporter plasmid (μg): expression plasmids (μg) = 1:0, 0.1, 0.5, and 1]. The cells were then cultured for various time intervals (6–48 h) (time-course experiment) or for 48 h without or with TO-901317 (1 μmol/L) (dose-response experiment). *P < 0.05 vs. value at time zero [0 (open bars)] or corresponding control (C; empty plasmid). C: Combination experiment. HuH7 cells were transfected with PFK2/FBP2-Luc, LXRα/RXRα expression plasmids, and SREBP1c (S), LXRβ (Lβ), or ChREBP/Mlx (Ch) expression plasmid(s) [reporter plasmid (μg): expression plasmid(s) (μg) = 1:1], and then the cells were cultured for 48 h. *P < 0.05 vs. corresponding control [C (open bars); empty plasmid]. #P < 0.05 vs. LXRα/RXRα group (Lα). In all figures, hatched, dotted, or closed bars represent values of treated groups.