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. Author manuscript; available in PMC: 2012 Apr 20.

Published in final edited form as: J Neurovirol. 2011 May 10;17(4):382–392. doi: 10.1007/s13365-011-0035-4

Fig. 1 — The role of osteopontin in HIV replication. a Quantitative PCR for SPP1 and 18S rRNA message was performed on cDNA prepared from HIV-1 SF162, and BaL and infected macrophages and controls. A representative of two independent experiments with mean and standard deviation with three different donors is shown. b Western blot analysis for OPN and actin 48 h after transfection of macrophages with anti-OPN siRNAs (SPP1, 3, 4, 6). c Scatter plots and graph: quantification of GFP⁺ cells by flow cytometry. A representative of two independent experiments with mean and standard deviation with three different donors is shown. d Western blot analysis for OPN on uninfected TZM-bl cells or cells infected with HIV and recombinant adenovirus expressing OPN or red fluorescent protein (RFP). e Increased replication and syncytia formation in TZM-OPN-expressing cells infected with HIVR3Nef+GFP by fluorescent microscopy and f quantified by flow cytometry (top graph). The mean fluorescent intensity (MFI) of GFP expression in HIV-infected cells or HIV-infected cells over-expressing OPN is shown in the middle graph. Activation of the HIV-LTR-beta-galactosidase promoter is shown in the bottom graph. Means and standard deviations are shown

Fig. 1 — The role of osteopontin in HIV replication. a Quantitative PCR for SPP1 and 18S rRNA message was performed on cDNA prepared from HIV-1 SF162, and BaL and infected macrophages and controls. A representative of two independent experiments with mean and standard deviation with three different donors is shown. b Western blot analysis for OPN and actin 48 h after transfection of macrophages with anti-OPN siRNAs (SPP1, 3, 4, 6). c Scatter plots and graph: quantification of GFP⁺ cells by flow cytometry. A representative of two independent experiments with mean and standard deviation with three different donors is shown. d Western blot analysis for OPN on uninfected TZM-bl cells or cells infected with HIV and recombinant adenovirus expressing OPN or red fluorescent protein (RFP). e Increased replication and syncytia formation in TZM-OPN-expressing cells infected with HIVR3Nef+GFP by fluorescent microscopy and f quantified by flow cytometry (top graph). The mean fluorescent intensity (MFI) of GFP expression in HIV-infected cells or HIV-infected cells over-expressing OPN is shown in the middle graph. Activation of the HIV-LTR-beta-galactosidase promoter is shown in the bottom graph. Means and standard deviations are shown