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. Author manuscript; available in PMC: 2012 Apr 20.
Published in final edited form as: J Neurovirol. 2011 May 10;17(4):382–392. doi: 10.1007/s13365-011-0035-4

Fig. 2.

Fig. 2

Single-cell analyses reveal that the NF-κB inhibitor IκBα is decreased in HIV-infected cells expressing OPN. TZM-bl cells infected with HIVR3Nef+GFP (green) were fixed and immunostained for IκBα (red), nuclei with DRAQ5 (blue). a TZM-bl cells only; b TZM-bl infected with Ad-OPN; (c) HIV-infected TZM-bl; d HIV-infected TZM-bl expressing OPN. The relative normalized pixel intensity of IκBα is shown (top right graph). All pictures were taken on a LSM 510 Meta at the same pinhole and intensity settings. The means and standard deviations of a representative of two independent experiments are shown. Western blot analysis for IκBα is shown in the middle panel and quantification shown in the bottom graph: HIV-infected TZM-bl cells only (HIV) or co-infected with Ad-RFP (HIVRFP) or Ad-OPN (HIVOPN); HIV-uninfected cells and exposed to Ad-RFP (RFP) or Ad-OPN (OPN). A representative of two independent experiments is shown