Fig. 4.

OPN is increased in the CSF and brain of HIV-infected persons. a Samples were grouped by MSK with MSK 0 (n=21), representing normal cognition and MSK 0.5 (n=35), MSK 1 (n=33) and MSK 2 (n=13) representing increasing severity of cognitive impairment; population means are indicated by hash mark. Inflammatory (n=30) and non-inflammatory (n=27) CSF samples were obtained from HIV-uninfected individuals (Table 1). Data was analyzed with one-way ANOVA, Dunn’s multiple comparison post test: *p<0.05, **p<0.01, ***p<0.001. b Osteopontin is increased in brain tissue of HIV-infected persons with cognitive disorder. Cell lysates prepared from frozen brain tissue from HIV-infected patients with stable neuropsychological diagnoses from the NNTC (Table 2, supplemental data) were analyzed by Western blot for OPN and β3-tubulin and quantified (c). HAD HIV-associated dementia, MCMD minor cognitive motor disorder, Sub subsyndromic, Normal neurocognitively normal, Cont control TZM-bl lysate. The Westerns were also probed for the HIV Nef protein. A representative of three independent experiments is shown