Table 1.
Antibodies used for IHC with method information.
| Antibody | Clone | Species* | Source | Pre-Treatment ** | Detection System *** | Dil. (1:X) |
|---|---|---|---|---|---|---|
| CD3 | SP7 | RM | Thermo Scientific | TUF (30′) | RPV | 300 |
| CD4 | 4B12 | MM | Vector lab | EDTA (50′) | MPV | 200 |
| CD8 alpha (human) | C8/144B | MM | Dako | EDTA (30′) | MEV | 100 |
| CD8 (monkey) | VP-C325 Clone 1A5 | MM | Vector lab | Trilogy Solution | MEV | 50 |
| CD8 beta (human) | F-5 | MM | Santa Cruz (sc-25277) | EDTA (30′) | MEV | 50 |
| CD14 | 7 | MM | Abcam (ab49755) | EDTA | MEV | 100 |
| CD19 | LE-CD19 | MM | Serotec (MCA24254T) | Citrate | MEV | 50 |
| CD20 | L26 | MM | Dako (N1502) | Dako RTU | MEV | RTUb |
| CD21 | IF8 | MM | Dako (M0784) | DAKO PTS (PC 3′) | MPV | 40 |
| CD31 | JC/70A | MM | Dako | EDTA (30′) | MPV | 100 |
| CD34 | QBEnd-10 | MM | Beckman-Coulter | Citrate | MPV | 70 |
| CD35 | Ber-MARC-DRC | MM; RMa | Dako | DAKO PTS (PC 3′) | RPV | 30 |
| CD45 | 2B11+PD7/26 | MM | Dako | Citrate | MPV | 500 |
| CD56 | 123C3.D5 | MM | Cell Marque | EDTA (30′) | MPV | 50 |
| CD68 | PGM1 | MM | Dako (M0876) | Dako RTU | MEV | 200 |
| CD163 | 10D6 | MM | NovoCastra | DAKO PTS (PC 3′) | MPV | 400 |
| CD172a | Human (syn. peptide) | RP | Abcam (ab53721) | Citrate | REV | 2000 |
| CD234 (Darc) | 358307 | MM | R&D Systems (MAB4139) | Citrate | MEV | 150 |
| D2-40 | D2-40 | MM | Covance | Citrate (PC) | MPV | 100 |
| FHOD-1 | Human protein (full-length, aa1-1165) Human (N-term aa 1-965) |
MP RP |
Abcam (ab73443) Fingeroth lab |
Citrate | MEV | 200 |
| TSP1 | A6.1 | MM | Abcam ab1823 | Citrate (PC) | MEV | 25 |
*
RM = Rabbit Monoclonal; RP = Rabbit Polyclonal; MM = Mouse Monoclonal; MP = Mouse Polyclonal
**
TUF = Tissue Unmasking Fluid; PTS = Pre-treatment solution (proprietary); PC = Pressure Cooker
***
RPV = Rabbit Powervision; MPV = Mouse Powervision; REV = Rabbit Envision; MEV = Mouse Envision
a
A mouse-anti-human is used as primary followed by a rabbit-anti-mouse and then Rabbit Powervision
b
Ready to Use
c
A rabbit antisera to the N terminal portion of FHOD-1 was also used in some assays.