Table 1.
Oligonucleotide primers for amplification and modification of antibody V-regions
| Primer Name | Sequencea |
|---|---|
| First Round Primers | |
| M-IgG2a | 5′ ATTCGGATAGATCTAGTGGATAGACCGATGG 3′ |
| MK-REV | 5′ ATTCGGATAGATCTTGGATGGTGGGAAGATG 3′ |
| MHV-5 | 5′ ACTAGTCGACATGGACTCCAGGCTCAAT TTAGTTTTCCTT 3′ |
| MKV-4 | 5′ ACTAGTCGACATGAGGGCCCCTGCTCACTTTTTTGGCTTCTTG 3′ |
| Second Round Primers | |
| VK-5′ | 5′ TCACGAAGTCTAGACTGGACATGAGGGCCCCTGCTCACTTTTTTGG 3′ |
| VK-3′ | 5′ GAATCTATGGATCCTGACACACTTACGCTTTATTTCCAACTTTGTCCCCGA 3′ |
| VH-5′ | 5′ ACACTATACTCGAGCTCACGATGAACTTTGGGCTCAGCTTGATTT 3′ |
| VH-3′ | 5′ TTCAGATCAAGCTTGACACACTTACCTGAGGAGACGGTGACTGAGGTTC 3′ |
a
Restriction endonuclease sites are underlined