The WNV protease crystal structure coordinates (PDB ID code 2FP7) were used for molecular docking. AutoDock Vina was used to dock each compound in the active site bound by Bz-Nle-Lys-Arg-Arg-H substrate-based inhibitor (Erbel et al., 2006). The solvent-accessible surface of the protein is shown in color-coded atoms (white, carbon; red, oxygen; blue, nitrogen). The catalytic triad (H51, D75, and S135) is shown as a green surface. A. The structure of the Bz-Nle-Lys-Arg-Arg-H substrate-based inhibitor bound WNV NS2B-NS3pro (Erbel et al., 2006). B. Compound 14 is shown in the orientation posed by molecular docking. Compounds 17, 18, 22 and 26 are shown in panels C, D, E and F, respectively.