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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Immunol. 2012 Apr 2;188(9):4376–4384. doi: 10.4049/jimmunol.1101775

Figure 2. Exogenous SP-A stimulation enhances the generation of Foxp3+ cells.

Figure 2

Purified T cells were isolated from C3BFeJ mice (2×10^6 cells/ml, allo T cells), stimulated with irradiated splenocytes from C57Bl/6J mice (5×10^6 cells/ml) and cultured for 4 days in the absence or presence of 20 μg/ml of exogenous human SP-A in one representative of three independent experiments with at least 3 mice per group (B). Tregs were defined as CD3+CD4+CD25+Foxp3+ cells and were characterized by flow cytometry (A). Purified T cells from spleen or lungs of WT or SP-A−/− mice were activated with anti-CD3 (1.5 μg/ml) and anti-CD28 (0.5 μg/ml) for ~3 days in the presence of SP-A(20 μg/ml), SP-D (5 μg/ml), C1q (20μg/ml) or control Ig(20 μg/ml)(C and D). The Foxp3+ population was assayed by flow cytometry, represented as initial frequency (white bars), after activation (gray bars), or after activation in the presence of SP-A (black bars) or SP-D or C1q (stipled bars). All data is normalized to initial levels of Foxp3+ T cells in the spleen. * p<0.05, ** p<0.01 compared to initial levels of Foxp3+ cells; ^ p<0.05 compared to post-activation levels in WT mice; #p<0.03 for post-activation SP-A mediated increase over post-activation + Ig conditions, in both WT and SP-A−/− mice, across four independent experiments.