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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Nat Chem Biol. 2012 Apr 1;8(5):477–485. doi: 10.1038/nchembio.926

Figure 5. Auxin Agonists Differentially Stabilize TIR1-Aux/IAA Complexes.

Figure 5

a. IAA, 1-NAA, and 2,4-D enhance TIR1-IAA7 interaction in yeast. Diploids carrying LexA DBD-TIR1 and AD-IAA7 were spotted on selective media containing 50 μM auxins. β-galactosidase expression is a measure of TIR1-IAA7 interaction. b. Surface Plasmon Resonance analysis of auxin-dependent TIR1-IAA7 DII interaction. Sensorgram shows the effect of no auxin (brown) and various auxinic compounds (500 μM IAA (dark blue), 50 μM IAA (light green), 50 μM 1-NAA (light blue), 50 μM 2,4-D (purple), 50 μM picloram (pink), 50 μM tryptophan (orange)) on TIR1-DII peptide association and dissociation. Auxins were mixed with TIR1 in solution prior to injection over DII peptide. c and d. Competitive [3H] IAA binding assays using 1-NAA and 2,4-D cold competitors. TIR1-IAA7 co-receptor complex binds 1-NAA and 2,4-D with a Ki (inhibition constant) of 113.50 ± 3.51 nM and > 1 μM, respectively.