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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: J Immunol. 2012 Mar 28;188(9):4385–4393. doi: 10.4049/jimmunol.1101116

Figure 1. PSGL-1 is functionally important for homing of the BM progenitors.

Figure 1

(A) Mixed BM chimeras were generated using CD45.2 PSGL-1 WT BM and CD45.1 WT BM mixtures as controls (top panels), or CD45.2 PSGL-1 KO BM and CD45.1 WT BM mixtures (bottom panels). Chimeras were analyzed by flow cytometry after 10 weeks using antibodies to CD45.1 and CD45.2 to determine donor chimerism. Representative FACS profiles of indicated progenitors in the BM and Thymus are shown. (B) Data are mean CD45.2 donor chimerism ± S.E.M. for each indicated population in mixed BM chimeras described in (A). ***P<0.001 for the CD45.2 donor chimerism of the indicated population compared with HSC CD45.2 donor chimerism. Numbers are from 5 mice per group, and are representative of 3 independent experiments that gave similar results. (C) Sorted LMPP from WT or PSGL-1 KO mice were cultured on OP9 (top panel) or OP9-DL4 (bottom panel) stromal layers. Both cells were cultured in triplicate wells. After 2 weeks, cultured cells were analyzed by flow cytometry for expression of CD25 and Thy1.2. Representative FACS plots of cells gated for CD45 expression are shown. (D) Graph show the total numbers of cells obtained from the cultures described in panel C. Data are mean ± S.E.M. of triplicate wells.