Figure 5. Glycosyltransferase enzyme gene expression profile in BM and early thymic progenitors.

Quantitative PCR analysis of total cellular RNA from indicated cell fractions purified from Gr-1, CD19, Mac-1 depleted BM and CD4, CD8 depleted thymus of C57BL/6 WT mice. We identified LT-HSC as CD150⁺CD34⁻ and ST-HSC as CD150⁻CD34⁺ cells among LSK BM progenitors. MPP were LSKFlt3^lo, CCR9⁺LP were LSKFlt3^hiCCR9^hi, Ly6D⁻CLP were Lin⁻Sca1^loKit^loFlt3^hiIL7Rα⁺Ly6D⁻ and Ly6D⁺CLP were Lin⁻Sca1^loKit^loFlt3^hiIL7Rα⁺ Ly6D⁺ cells in BM. To isolate thymic progenitors, we identified Flt3⁺ETP as Lin⁻Kit⁺CD25⁻Flt3⁺, Flt3⁻ETP as Lin⁻Kit⁺CD25⁻Flt3⁻, and DN3 as Lin⁻Kit^loCD25⁺ cells in thymus. Transcript levels of indicated genes were normalized to GAPDH mRNA levels and are indicated in arbitrary units. Means and standard errors of the results obtained from 3 independent measurements are shown.