Figure 6. Resveratrol inhibits IL-2-induced expansion of Treg.

Splenocytes and infiltrating cells were stained with FITC-conjugated anti-CD4 mAb, fixed and permeabilized and incubated with PE-conjugated anti-FoxP3 mAb as described in Materials and Methods. The stained cells were analyzed by flow cytometry. The representative dot plots from spleen (A), lung (B), and liver (C) and the statistical analysis of the percentage mean ±SEM of Treg (D) are shown.