Figure 5. Absorbance Spectra of Cobalamin bound to Purified Zebrafish Cobalamin Binder.
Spectral characteristics of the zebrafish cobalamin binder in complex with hydroxo-cobalamin are shown. Spectral transition after addition of 15 mM histidine was recorded after 1, 3, 5, 12, 20, and 30 minutes of incubation at room temperature. The arrows indicate the direction of this transition.