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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: Dev Cell. 2012 Apr 17;22(4):799–810. doi: 10.1016/j.devcel.2012.02.006

Figure 6. Missense mutations in HDS1 cause defects in growth and TGN localization synthetic with arf1Δ.

Figure 6

(A) Plasmids were introduced into a Sec7 shuffling strain (CFY409) and assayed for the ability to complement a sec7Δ null mutant, monitored by growth on media containing 5-FOA. Vector (pRS415), HA-Sec7 (pCF1101) and HA-Sec7ΔHDS1 (pCF1139) were tested.

(B) Plasmid-borne GFP-sec7 alleles with the indicated residues substituted with alanine were tested for their ability to complement sec7Δ arf1Δ (CFY863). Plates were imaged after 3 days of growth at the indicated temperature.

(C) GFP-tagged sec7 alleles with the indicated HDS1 alanine substitutions were imaged in log phase sec7Δ arf1Δ cells (CFY863). The GFP signal of the ExxKN1154 mutant was consistently brighter, suggesting cells require multiple copies of the plasmid for viability.

See also Figures S5, S6, and S7.