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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: Cancer Cell. 2012 Apr 17;21(4):532–546. doi: 10.1016/j.ccr.2012.02.019

Figure 7. VHL-induced expression of LC3C protects RCC cells from inhibition of LC3B-dependent autophagy.

Figure 7

(A) Western blot showing time course of LC3C-II induction in VHL(+) and VHL(−) 786-O and A498 cells in response to serum starvation.

(B) Quantitative RT-PCR for LC3C and LC3B mRNAs (48 hr of starvation) in VHL(+) as compared with VHL(−) cells.

(C) Quantitative RT-PCR for LC3C mRNA in VHL(−) cells where HIF-2α was knocked down by using siRNA.

(D) Quantitative RT-PCR for LC3C mRNA in VHL(+) cells where HIF was stimulated with DMOG (1 mM) for 16 hr.

(E) Effect of single LC3C or LC3B knockdown or of double LC3C/LC3B knockdown on the viability of indicated cells, as compared with scramble-treated cells. The timeline followed was the same as that shown in Figure 3A. See also Figure S7.