Table 2. PCR primer pairs used for DNA methylation analysis.
| Fragment | Primer Sequencea | Amplicon size (bp) | Target sequence |
| I | TATTGGGTGGAAATATTTAGGTTTG | 279 | LoxP and start of TK promoter |
| TACCTCAACCATAAAAACAAACACA | |||
| II | GGATAGTAAGGGGGAGGATTG | 520 | Part of TK promoter and start of EGFP |
| CAAATAAACTTCAAAATCAACTTACC | |||
| III | GGGGTTTATTTAGGAAAATGAAATG | 301 | End of SV40 pA and lox2272 |
| TCCCCCTAAACCTAAAACATAAAATA | |||
| BCP | TAAAAATTTTGGGGAGTATTTTAAAGG | 461 | β-casein promoter |
| TAACAATCCACCAAACTCCACTATT | |||
| GR | TTTTTTTGAAGTTTTTTTAGAGGG | 324 | Glucocorticoid receptor |
| AATTTTCTCTATAATTTCTCTTCTTACC | |||
| Sat 1 | TGTAGATTGGGGATAGGAGAGTTAG | 345 | Satellite 1 |
| CCTACTTTATCTAAAAAAAATTACCTTCC |
a
all forward primers (top) included the following common sequence at the 5′ end: AGGAAGAGAG; all reverse primers (bottom) the sequence CAGTAATACGACTCACTATAGGGAGAAGGCT.