Figure 4. Apoptosis assessment of B16-F10 melanoma cells submitted to a heat treatment (HT), 45°C for 30 min.

Representative histograms (from four independent experiments), before the heat treatment (d 0) and after, at defined time-points (d 0, 0 h–d 14, immediately to 14 days after heat exposure). Flow cytometry analysis of Annexin-FITC/PI uptake (A, necrosis; B, late apoptosis; C, viable cells; D, early apoptosis).