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. 2012 Apr 20;7(4):e35746. doi: 10.1371/journal.pone.0035746

Figure 4. Trans-activation of the exon 1a promoter by YY1.

Figure 4

(A) and (B) HeLa cells were co-transfected with 100 ng of the exon 1a reporter plasmid (−106/+266-Luc) along with 400 ng of the plasmid containing YY1 expression plasmid, or 200 ng of promoter constructs with 300 ng of the dominant-negative mutant YY1S339/S342 as indicated. (C) Effect of mutation of YY1 binding sites on YY1 induction of luciferase activity under the control of the exon 1a promoter. 100 ng of the exon 1a mutants containing mutations as described in Fig. 2B and Table 1 were transfected into HeLa cells with or without 400 ng of the YY1 construct. Luciferase activity was measured 24 h after transfection. Means ± S.E.M. are shown (n = 4). *P<0.01 versus −106/+266; **P<0.01 versus −106/+266 + YY1; #P<0.05; ##P<0.01.