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. 2012 Apr 20;7(4):e34256. doi: 10.1371/journal.pone.0034256

Table 3. Upregulation of transcript abundance by CTR9 RNAi.

geneID rpkm CTR9/WT features
Procyclin locus
Tb927.6.520 EP3-2 procyclin 36.4 17 pol I
Tb927.6.510 GPEET2 procyclin 31.3 14 pol I
Tb927.10.10240 PAG1 2.5 48 pol I
Tb927.10.10220 PAG2 1.9 57 pol I
Tb927.6.530 PAG3 12.2 11 pol I
Tb927.10.10210 PAG4 2.6 31 pol I
Tb927.10.10230 PAG5 2.3 37 pol I
Near rRNA locus
Tb09.v2.0450 Unlikely ORF 2.0 17.0 rRNA locus
Tb927.2.1470 hypothetical conserved 3.6 10.0 0 kb, rRNA
Tb927.1.3750 hypothetical conserved 8.7 7.7 0 kb, rRNA
Tb927.3.3430 hypothetical conserved 7.4 7.6 0 kb, rRNA
Other
Tb927.5.4020 hypothetical 4.0 13** 3 kb
Tb927.8.6570 hypothetical conserved 3.4 10 0 kb
Tb927.8.510 hypothetical 1.1 21* 6 kb
Tb927.10.4390 hypothetical 3.6 7.8 3 kb
Tb927.10.4380 hypothetical 0.8 39* 2 kb, ESAG
Tb927.10.12270 hypothetical conserved 4.1 14* 1 kb
Tb927.10.1760 hypothetical 1.7 20* 0 kb
Tb927.11.9380 hypothetical 1.1 14* 0 kb
Tb927.1.2020 hypothetical 3.3 11 0 kb
Tb927.11.11990 hypothetical 1.3 11 0 kb
Tb927.8.480 phosphatase 26.6 9.8 0 kb
Tb927.2.3330 hypothetical 5.3 8.7 0 kb
Tb927.7.310 hypothetical conserved 8.9 8.1 0 kb
Tb927.10.9550 hypothetical 0.6 8.4 6 kb
Tb927.8.5930 hypothetical 0.3 9.4 stop/start
Tb927.3.650 hypothetical conserved 25.8 8.1 11 kb
Tb927.7.2430 Long poly(AT) tracts 0.2 16.9* repetitive
Tb927.11.4600 hypothetical 1.0 18.5 None
Tb927.10.10040 hypothetical 4.1 13.3 None
Tb927.3.2880 hypothetical conserved 7.6 11.7 None
Tb927.10.9030 hypothetical conserved 10.7 9.7 None
Tb927.3.3570 hypothetical conserved 10.8 9.1 None
Tb927.11.7260 hypothetical conserved 7.8 8.7 None
Tb927.6.4790 hypothetical conserved 33.9 8.2 None
Tb11.v4.0003 hypothetical 2.3 8.2 None
Tb07.26A24.210 hypothetical 4.7 7.8 None
Tb927.3.2370 hypothetical conserved 36.0 7.8 None
Tb927.10.260 hypothetical conserved 29.4 7.7 None
Tb927.10.7720 hypothetical conserved 35.7 7.6 None
Tb927.11.12330 hypothetical conserved 24.5 7.5 None
Tb927.2.2070 hypothetical 3.5 8.6
Tb927.6.4570 hypothetical conserved 13.8 8.6 start

One representative of each of the procyclin locus ORFs is shown, along with the 36 other ORFs showing the greatest increases in RNA after CTR9 RNAi. “rpkm” is the reads per kilobase per million reads seen in poly(A)+RNA from normal (wild-type) cells, taken from [30]; the median for all ORFs is 30.6. “CTR9/WT” shows the fold change in rpkm after RNAi (2 significant figures); **no signal was seen on a Northern blot using an ORF probe; *no RNA detected by reverse transcription and PCR targeting the ORF. “Features” indicates transcription by polymerase I (pol I), and the distance of the 3′ end of the locus mRNA from a convergent transcription stop area – “0 kb” indicates that this is the last ORF in a transcription unit. “0 kb, rRNA” indicats that the ORF is the last on a pol II transcription unit, next to an rRNA locus read in the opposite direction. “2 kb, ESAG” is a gene upstream of an ESAG. An rpkm of 20 corresponds to 1 mRNA per cell, which is difficult to detect by Northern blotting.