Table 3.

Second order rate constant for incorporation of d5SICSTP against X in the template by different polymerases.^[a]

Polymerase	X	kcat/KM (M−1 min−1)
Kf(exo−)	dMMO2	4.7 × 107 [b]
	dTMO	2.7 × 107
	dFMO	4.9 × 105
Taq	dMMO2	3.5 × 106 [c]
	dTMO	6.4 × 106
	dFMO	1.8 × 104
Vent(exo−)	dMMO2	9.9 × 106 [c]
	dTMO	5.5 × 106
	dFMO	1.1 × 105

^[a]See experimental section for experimental details.

^[b]Taken from reference [8].

^[c]Taken from reference [13].