Table 4.
Template | Base pair amplified | Amplification | Fidelity[b] |
---|---|---|---|
D1[c] | dMMO2-d5SICS | 224 | 99.4 |
D1 | dDMO-d5SICS | 397 | 99.8 |
D1 | dTMO-d5SICS | 364 | 99.1 |
D1 | dFMO-d5SICS | 121 | 91.9 |
D7[c] | dA-dT | 556 | - |
D2 | dDMO-d5SICS | 69 | 95.7 |
D3 | dDMO-d5SICS | 130 | 97.9 |
D4 | dDMO-d5SICS | 78 | 90.7 |
D5[c] | dMMO2-d5SICS | 25 | 97.1 |
D5 | dDMO-d5SICS | 12 | 94.4 |
D6[c] | dMMO2-d5SICS | 52 | 92.9 |
D6 | dDMO-d5SICS | 109 | 97.6 |
Conditions: 1 ng of the DNA template; dNTPs/dXTPs = 600/400 μM, 6 mM MgSO4, 0.03 U/μL of the enzyme, 8 min extension, 14 cycles.
Calculated from sequencing data as described in Supporting Information.
Taken from reference [15].