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. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Chemistry. 2010 Nov 8;16(42):12650–12659. doi: 10.1002/chem.201000959

Table 4.

PCR efficiency and fidelity.[a]

Template Base pair amplified Amplification Fidelity[b]
D1[c] dMMO2-d5SICS 224 99.4
D1 dDMO-d5SICS 397 99.8
D1 dTMO-d5SICS 364 99.1
D1 dFMO-d5SICS 121 91.9
D7[c] dA-dT 556 -

D2 dDMO-d5SICS 69 95.7
D3 dDMO-d5SICS 130 97.9
D4 dDMO-d5SICS 78 90.7
D5[c] dMMO2-d5SICS 25 97.1
D5 dDMO-d5SICS 12 94.4
D6[c] dMMO2-d5SICS 52 92.9
D6 dDMO-d5SICS 109 97.6
[a]

Conditions: 1 ng of the DNA template; dNTPs/dXTPs = 600/400 μM, 6 mM MgSO4, 0.03 U/μL of the enzyme, 8 min extension, 14 cycles.

[b]

Calculated from sequencing data as described in Supporting Information.

[c]

Taken from reference [15].