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. Author manuscript; available in PMC: 2013 Mar 20.
Published in final edited form as: Biochemistry. 2012 Mar 8;51(11):2213–2223. doi: 10.1021/bi300123a

FIGURE 4.

FIGURE 4

1H-15N HSQC spectra as a function of pH. A. Overlay of 1H-15N HSQC spectra collected at pH 5.5 (black), 6.0 (green), 6.5 (blue), 7.0 (orange), and 7.5 (red). The chemical shifts of several peaks are affected by pH whereas others do not change. The chemical shifts of the three histidine residues in the FAT domain (H981, H1026, and H1053) are circled. B. The weighted changes in chemical shift from pH 5.5 to 7.5 were calculated. Changes greater than 0.05 ppm (dashed line) were considered significant. The histidine residues are shown in red.