Table 2.
Sample | Rg(nm) | Dmax(nm) | Ve(nm3) | Vp (nm3) | χg | χx | χn | χo | χb | χf |
---|---|---|---|---|---|---|---|---|---|---|
Yfh1wt Yfh1wt + glycerol |
1.8 2.25 |
6.8 7.5 |
28 32.2 |
24 44 |
1.16 1.36 |
1.19 1.2 |
1.16 3.26 |
1.03 2.53 |
1.04 1.4 |
1.02 1.01 |
Rg(nm), Dmax, Ve(nm3) and Vp(nm3) are radius of gyration, maximum size, excluded volume, and Porod volume, respectively. χg, discrepancy of GASBOR ab initio models; χx, discrepancy to new X-ray structure; χn, discrepancy to lowest-energy NMR conformer (model #1 in 2GA5.PDB); χb, discrepancy to the BUNCH model; χo, OLIGOMER discrepancy to twenty NMR conformers and X-ray model; χf, discrepancy to EOM ensemble model.