Figure 3. Effect of expressing PFK2/FBPase2 point mutants on islet Ca²⁺ oscillations.

(A) Representative recordings of islets Ca²⁺ in the presence of the indicated constructs and 1 µM Shield1. (B and C) Average oscillatory period (B) and amplitude (C) were determined from >20 min of intracellular Ca²⁺ measurements of transduced islets treated with vehicle or Shield1. Numbers in parentheses indicate the number of islets measured in ≥4 independent experiments. 11.1 mM glucose was used in all experiments. * p<0.05, ** p<0.01, *** p<0.001.