Skip to main content
. Author manuscript; available in PMC: 2012 Apr 22.
Published in final edited form as: Nat Med. 2011 Nov 13;17(12):1610–1618. doi: 10.1038/nm.2506

Figure 5.

Figure 5

Characterization of inflammatory and fibrotic response between AC3-I and WT mice after MI + Aldo. (a) The number of MPO positive cells identified on immunohistochemistry of heart tissue sections is similar between WT and AC3-I mice 24 hours after MI + Aldo and between ruptured and non-ruptured WT MI + Aldo mice. P < 0.001, One-way ANOVA, n ≥ 3 mice per group, ***P < 0.001, Bonferroni's multiple comparison test. (b) MPO activity is similar between WT and AC3-I mice. MPO activity increases after MI in both WT and AC3-I mice. AC3-I mice show no difference to WT mice even after MI + Aldo. P = 0.001, One-way ANOVA, n ≥ 3 mice per group, *P < 0.05, Bonferroni's multiple comparison test. (c) Cumulative Mac-3 staining is similar in AC3-I mice compared to WT mice 3 days after MI + Aldo. n ≥ 3 mice per group. (d) Differential regulation of pro-fibrotic genes after MI + Aldo in WT and AC3-I mice, Ctgf = connective tissue growth factor, Col1a2 = collagen type I alpha 2, Col3a1 = collagen type III alpha 1, n ≥ 4 mice per group, One-way ANOVA, ***P < 0.001, Bonferroni's multiple comparison test. (e) Masson's trichrome staining shows similar increase in fibrosis after MI + Aldo in both WT and AC3-I. Student's t-test versus MI + Veh, n ≥ 3 mice per group. Black scale bar = 2 mm. White scale bar = 50 μm.