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. 2012 Apr 20;7(4):e35354. doi: 10.1371/journal.pone.0035354

Figure 3. Effect of glutamate on glutamine synthetase and glutamate transporter gene expression in MeCP2-null astrocytes.

Figure 3

A–C. Effects of Glu on Glu clearance-related genes in wild-type (white column) and MeCP2-null (gray column) astrocytes. Semi-quantitative RT-PCR analysis of Glu clearance-related genes, EAAT1 (A), EAAT2 (B), and GS (C), was performed in the control and MeCP2-null astrocytes 12 or 24 h after treatment with 1.0 mM Glu. The bands corresponding to PCR products were quantified by densitometry, normalized against HPRT levels, and expressed as % of controls (equals 100%). Bars represent the means ± SE of samples from 3–4 independent experiments (*p<0.05, **p<0.01). D–F. Comparison of the effects of Glu on EAAT1, EAAT2 or GS expression in the control and MeCP2-null astrocytes. The ratio of EAAT1/HPRT (D), EAAT2/HPRT (E) or GS/HPRT (F) in each treatment group was normalized against that of the non-treated astrocytes from each group. Bars represent the means ± SE of samples from 3–5 independent experiments (*p<0.05). Numbers in each column indicate the total number of samples analyzed.