Table 1. PCR primers.
Sense | Antisense | Ta | cycles | |
GFAP | 5′-ATCCGCTCAGGTCATCTTACCC-3′ | 5′-TGTCTGCTCAATGTCTTCCCTACC-3′ | 63 | 25 |
S100β | 5′-AGAGGACTCCAGCAGCAAAGG-3′ | 5′-AGAGAGCTCAGCTCCTTCGAG-3′ | 59 | 32 |
EAAT1 | 5′-GAAGTCTCCCAGACGTTCTAATCC-3′ | 5′-GCTCTGAAACCGCCACTTACTATC-3′ | 65 | 35 |
EAAT2 | 5′-ATGCTCATCCTCCCTCTTATCATC-3′ | 5′-CTTTCTTTGTCACTGTCTGAATCTG-3′ | 63 | 32 |
GS | 5′-TGTACCTCCATCCTGTTGCC-3′ | 5′-GTCCCCGTAATCTTGACTCC-3′ | 57 | 25 |
HPRT | 5′-CCTGCTGGATTACATTAAAGCACTG-3′ | 5′-AAGGGCATATCCAACAACAA-3′ | 57 | 30 |
MeCP2 | 5′-GGTAAAACCCGTCCGGAAAATG-3′ | 5′-TTCAGTGGCTTGTCTCTGAG-3′ | 61 | 35 |
GFAP, glial fibrillary acidic protein; EAAT, excitatory amino acid transporter; GS, glutamine synthetase; HPRT, hypoxantine-phosphoribosyl-transferase; MeCP2, methyl-CpG-binding protein 2; Ta, annealing temperature (°C).