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. Author manuscript; available in PMC: 2012 Apr 23.
Published in final edited form as: Circ Res. 2009 Mar 5;104(7):879–886. doi: 10.1161/CIRCRESAHA.108.193102

Figure 6.

Figure 6

MiR-199a is downregulated during IPC in porcine hearts and is associated with upregulation of Hif-1α and Sirt1. a, Porcine hearts were preconditioned via 2×10-minute cycles of ischemia/reperfusion of the left ventricle (n = 3). A second set of animals was subjected to a sham operation. The IPC area of the left ventricle, remote zone, and sham-operated ventricles were immediately dissected (early/first window IPC) and analyzed by Northern and Western blotting. The top 2 gels are the results of a Northern blot, and the lower 3 gels are Western blots. b, Cultured adult rat cardiac myocytes were treated with miR-199a eraser for 24 hours or HPC. Protein was extracted and analyzed by Western blotting for the molecules indicated (n = 3).