Table 2.
Intracellular IFN-γ Measurement of CD8+ T Cell in Response to Dominant Epitopes Targeted by Slow- and Rapid-Progressor Children over Time of Infection
| Participants | Optimal epitope | Epitope sequence | Early | Intermediate | Late |
|---|---|---|---|---|---|
| % IFN-γ+ CD8+ T cells | |||||
| Arm A (n=6) | |||||
| A-517C | B42-TL9 (Gag) | TPQDLNTML | 0.00 | 0.33 | 0.11 |
| CW3-YL9 (Gag) | YVDRFFKTL | 0.16 | 0.00 | 0.00 | |
| A-133C | B81-TL9 (Gag) | TPQDLNTML | 0.08 | 0.37 | 1.05 |
| A-298-C | A29-SY9 (Env) | SFDPIPIHY | 0.45 | 0.00 | 0.04 |
| A29-RW10 (Gag) | RQANFLGKIW | 0.02 | 0.00 | 0.12 | |
| A-562C | B58-TW10 (Gag) | TSTLQEQIAW | 0.16 | 0.06 | 0.07 |
| A-458C | CW3-YL9 (Gag) | YVDRFFKTL | 0.36 | 0.11 | 0.00 |
| A-447C | CW8-TL9 (Gag) | TPQDLNTML | 0.86 | 0.11 | 0.00 |
| Arm B (n=9) | |||||
| B-114C | CW7-KY11 (Nef) | KRQEILDLWVY | NDa | 0.16 | 2.35 |
| A23-RW8 (Nef) | RYPLTFGW | ND | 0.32 | 0.26 | |
| B-380C | B1510-GL9 (Gag) | GHQAAMQML | 0.00 | 0.46 | 1.70 |
| B1510-VL10 (Gag) | VHQAISPRTL | 0.00 | 0.13 | 0.20 | |
| A23-RW8 (Nef) | RYPLTFGW | 0.00 | 0.22 | 0.96 | |
| B-559C | B58-TW10 (Gag) | TSTLQEQIAW | 0.10 | 0.12 | 0.40 |
| CW7-KY11 (Nef) | KRQEILDLWVY | 0.00 | 3.10 | 0.96 | |
| B58-KW11 (Env) | KAYETEVHNVW | 0.14 | 1.56 | 0.25 | |
| B58-KAF9 (Nef) | KAAFDLSFF | 0.42 | 0.81 | ND | |
| B-586C | B81-TL9 (Gag) | TPQDLNTML | 0.05 | 0.64 | 0.52 |
| B-021C | A2-YI9 (Pol) | YTAFTIPSI | 0.06 | 2.18 | 0.08 |
| A29-SY9 (Env) | SFDPIPIHY | 0.28 | 1.93 | 0.16 | |
| B42-TL9 (Gag) | TPQDLNTML | 0.00 | 0.39 | 0.74 | |
| B-001C | B81-TL9 (Gag) | TPQDLNTML | 0.00 | 3.17 | 0.47 |
| B-222C | B7-TL10 (Nef) | TPGPGVRYPL | ND | 0.12 | ND |
| B-675C | CW3-YL9 (Gag) | YVDRFFKTL | 0.09 | 3.42 | 0.19 |
| B-732C | B45-GV11 (Nef) | GEVGFPVRPQV | 0.00 | 0.73 | ND |
| B7-RI10 (Env) | RPNNNTRKSI | 0.00 | 1.19 | ND | |
Dotted line separates slow-progressor from rapid-progressor children in both Arm A and Arm B groups. Terms in bold indicate time on ART as detailed in Table 1.
a
ND, not determined.
IFN-γ, interferon γ.