Figure 2. Characterization of Hpo.
(A) SDS-PAGE. M, molecular size markers; lanes 1 and 2, cell extracts of expressed Hpo in E. coli BL21(DE3) expressing His6-Hpo; lane 3, purified His6-Hpo. (B) pH dependence of Hpo specific activity. (C) Effect of varying temperatures on specific Hpo activity. (D) Effect of the various metal salts on Hpo specific activity. CK, without metal salt; Ni, Ni2+; Co, Co2+; Ca, Ca2+; Cu, Cu2+; Mn, Mn2+; Zn, Zn2+; Mg, Mg2+; Mo, MoO42−; W, WO42−; Fe, Fe2+.
