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. 1976 Nov;58(5):1233–1239. doi: 10.1172/JCI108577

Electrophysiological study of isolated perfused human collecting ducts: Ion dependency of the transepithelial potential difference.

H R Jacobson, J B Gross, S Kawamura, J D Waters, J P Kokko
PMCID: PMC333292  PMID: 993341

Abstract

Cortical and outer medullary collecting duct segments were dissected from human kidneys and perfused in vitro. The transepithelial potential difference was measured and found to be lumen positive +6.8 +/- 0.6 mV (n= 20). This lumen-positive potential difference was inhibited by ouabain and furosemide but not by acetazolamide. Replacement of chloride in bath and perfusion fluids caused a reversible decrease of the potential difference to near zero. We conclude from these studies: (a) the lumen-positive potential difference is dependent upon the presence of chloride ion suggesting the existence of an active electrogenic chloride reabsorptive process in the human collecting duct and (b) it is possible to examine human renal physiology directly using in vitro microperfusion of tubule segments.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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