Table 1.
Primer name | Target (CDS) | Sequence (5′–3′) | Amplicon size (bp) | Reference |
---|---|---|---|---|
EhVpol_F | DNA polymerase (ehv030) | TATAATGCACGCCAACTTGC | 98 | This study |
EhVpol_R | GCAATTGCACCAAGTGGATA | |||
EhVpcna_F | PCNA (ehv440) | GGGCATTTCATTTGCCATAC | 157 | This study |
EhVpcna_R | ATTCTCCGTCGACAATACGC | |||
EhVhel_F | Helicase (ehv104) | GCCAACTGGTACAGGGAAAA | 184 | This study |
EhVhel_R | CATCCATGCATGTGTGACAA | |||
MCP_F2 | MCP (ehv085) | GACCTTTAGGCCAGGGAG | 134 | Schroeder et al. (2002) |
MCP_R2 | GTTCGCGCTCGAGTCGAT |