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. 2011 Dec 20;40(8):3443–3455. doi: 10.1093/nar/gkr1234

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — (A) Summary of the data resulting from the HR-specific PCR assay presented in a previous study (16; values plotted in the leftward and middle columns [i.e. 0× nick and 1×nick, respectively]) and in the current work (values plotted in the rightward columns [i.e. 2× nick]). The results are shown in terms of the absolute frequencies of stably transfected (ST) cells (left graph, solid diamonds) and of AAVS1-targeted cells (left graph, open triangles) as well as in terms of the total numbers of reporter-positive clones analyzed (right graph, solid circles) and of those that were targeted at AAVS1 through HR (right graph, open squares). (B) Working model for nick-initiated mitotic HR in human cells (see text for details).