Figure 4.

DNase I footprints showing the interaction of P-TFO with REPSA-selected sequences 1–14 in the presence of 10 µM naphthylquinoline triplex-binding ligand. The experiments were performed in 50 mM sodium acetate pH 5.0; P-TFO concentrations (µM) are shown at the top of each gel lane. The lanes labelled ‘control’ show digestion of the DNA in the absence of oligonucleotide; tracks labelled ‘GA’ are markers specific for purines. The locations of the footprints are indicated by the filled boxes and any accompanying enhancements are indicated by asterisks.