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. 2011 Dec 22;40(8):3348–3363. doi: 10.1093/nar/gkr1244

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Rad26p does not alter the eviction of histone H3 from the coding sequences of GAL1 (A), GAL7 (B) and GAL10 (C) upon transcriptional induction. Both the wild-type and Δrad26 strains were grown in YPR up to an OD₆₀₀ of 0.9, and then switched to YPG for 30 min prior to formaldehyde treatment. Immunoprecipitation was performed using an anti-histone H3 antibody (Abcam; Ab-1791) against histone H3. Immunoprecipitated DNA was analyzed by PCR, using the primer pairs targeted to the coding sequences of GAL1, GAL7 and GAL10.