Figure 9.
Rad26p is predominantly associated with the coding sequences of CTT1 (A) and STL1 (B) following transcriptional induction. Yeast cells were grown in synthetic complete medium up to an OD600 of 0.9, and then induced by 0.45 M NaCl for 7 min prior to cross-linking. Immunoprecipitation was performed as described previously (13). Rad26p facilitates the loss of histone H2B from the INO1 (C), CTT1 (D), and STL1 (E) coding sequences following transcriptional induction. Immunoprecipitation was performed as in Figure 4.
