Figure 2.

Provenance of sdRNAs from within full-length box C/D snoRNAs in 14 diverse human cell types. (A) Relative abundance of the 5′ position of sdRNAs within full-length box C/D snoRNAs. To normalize for non-uniform distribution of snoRNA length, the 5′-ends of all box C/D sdRNAs detected in a given cell type were mapped and counted in 10% blocks of their respective full-length snoRNAs. For each cell type investigated, the abundance of sdRNAs mapped to a specific block was normalized by the total number of counts of all sdRNAs detected in that cell type and this relative abundance was plotted as a function of relative position within the full-length snoRNAs, as described in the ‘Materials and Methods’ section. (B) To investigate average sdRNA abundance as a function of snoRNA position for all snoRNAs individually, the relative abundance per block was calculated as above for each snoRNA and averaged over all snoRNAs. The error bars represent standard deviation. Only snoRNAs with sdRNA counts of at least 10 were considered for this analysis.