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. 2011 Dec 16;40(8):3763–3774. doi: 10.1093/nar/gkr1191

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Published by Oxford University Press, 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Plasmids and results for exploring different system composition approaches. (A) Constitutive T7 construct pKSGFP, and bicistronic constructs pCtltetKS, placI-GFP and pGFP-lacI. (B) Fluorescence after 10 h of EGFP expression from these constructs. (C) To investigate multi-plasmid systems, separate plasmids containing either GFP (pKSGFP) or lacI (pT7lacI) were constructed. (D) Results for co-expression of pKSGFP and pT7lacI for different percentages of pKSGFP by molar concentration. Error bars depict standard deviation of triplicate measurements.