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. 2011 Dec 16;40(8):3763–3774. doi: 10.1093/nar/gkr1191

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Published by Oxford University Press, 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Negative feedback system and results. (A) Negative feedback constructs pNFB-T7tet and pNFBtetKS. pNFB-T7tet has the ZE21 RBS for tetR and GFP along with the T1 terminator, while pNFBtetKS has the g10 RBS for tetR and GFP along with the T7 terminator. (B) Responses of pNFB-T7tet (red triangles) and pNFBtetKS (green squares) to different aTc concentrations. (C) Response dynamics of pNFB-T7tet with no aTc (open red triangles), pNFB-T7tet with 3300 ng/ml aTc (closed red triangles), pNFBtetKS with no aTc (open green squares), pNFBtetKS with 3300 ng/ml aTc (closed green squares). (D) Response dynamics of pNFBtetKS to different aTc concentrations in the PURE system. (E) Responses of E. coli BL21-AI harboring pNFBtetKS to inducer aTc.