Table 1.
Plasmids used for characterizing repression and for optimizing expression
| Plasmid | Promoter | RBS | Reporter | Backbone | Terminator |
|---|---|---|---|---|---|
| pT7 | T7 | ZE21 | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pT7tet | T7tet13 | ZE21 | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pT7tet2 | T7tet19 | ZE21 | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pT7tet-RBSII | T7tet13 | RBSII | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pT7tet-RBSg10 | T7tet13 | g10 | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pT7tet-RBS35 | T7tet13 | BBa_B0035 | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pT7tet-RBSA | T7tet13 | A | GFPmut3.1(ASV) | pZE21-MSC-2 | T1 |
| pUC-T7tet | T7tet13 | g10 | EGFP | pUC-19 | vsv |
| pUC-T7tet-T7term | T7tet13 | g10 | EGFP | pUC-19 | T7 |
| pT7tetKS | T7tet13 | g10 | EGFP | pBluescript-KS | T7 |
| pT7tetKS-SF | T7tet13 | g10 | EGFP | pBluescript-KS | T7 |
| pKSGFP | T7 | g10 | EGFP | pBluescript-KS | T7 |
| pLacOIDGFP | T7lacO1 | g10 | EGFP | pBluescript-KS | T7 |
| pLacOGFP | T7lacOID | g10 | EGFP | pBluescript-KS | T7 |
| pDEST17-EGFP | T7 | g10 | EGFP | pDEST-17 | T7 |