Figure 4.

Hypersensitivity of the rad50 and mre11 mutants to bleomycin. (A) Wild-type and mutant plants were inoculated as six explants into quadrants of plates containing standard growth medium with or without bleomycin at 8 ngml⁻¹. For the mutant strains, each inoculum represents an independent disruption line. The photograph illustrates the extent of growth 10 days following inoculation of the explants. (B) Survival curves of wild type and nbs1, mre11, rad50 and rad51 mutant protoplasts regenerating after exposure to bleomycin treatment. Error bars indicate SD based on at least two independent experiments in all cases.