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. 2011 Dec 21;40(8):3456–3469. doi: 10.1093/nar/gkr1242

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — The homeodomain of Cdx2 is necessary for its interaction with Ku70/Ku80. (A) Schematic representation of the different Cdx2 deletion mutants tested. TAD: transactivator domain; NLS: nuclear localization signal; HD: homeodomain; SD: stabilization domain. The upper numbers represents the position of the different domains in the 313aa long Cdx2 protein. (B) Coimmunoprecipitations of the different mutant proteins with Ku proteins. Co-IP was performed in HCT116 transfected with the indicated mutant plasmids using anti-Flag antibodies. Immunoprecipitates were separated on a SDS–PAGE and analyzed by western blot using anti-Ku70 and anti-Ku80 antibodies (upper panel), anti-Flag antibodies (middle panel). Ku70 and Ku80 proteins present in the protein extracts before immunoprecipitation were revealed by western blot (input, lower panel).